北京a-檢測(cè)底物5g愛(ài)爾蘭 （AZCL-Amylose Insoluble Chromogenic Substrates）
規(guī)格:5g
含量:98%
產(chǎn)地:愛(ài)爾蘭
外觀 黑色粉末
比重 不適用
溶解度 不溶于水，但水膨脹，從而形成凝膠
pH值 中性
氣味 無(wú)
形態(tài) 粉末
穩(wěn)定性 穩(wěn)定 室溫10年以上
北京a-檢測(cè)底物5g愛(ài)爾蘭 成分:
名稱 比例
AZCL-直鏈淀粉 98％
Dyed and crosslinked amylose （Sigma A-7043）. Substrate for the assay of a-amylase.
Catalogue Number: I-AZAMY
Content: 5 grams
Appearance Black powder
Specific Gravity Not applicable
Solubility in Water Not soluble, but swells in water to form a gel
pH Value Neutral
Odour None
Form Powder
Stability Stable at room temperature for ten or more years
Ingredients Name CAS Proportion
AZCL-Amylose 98%
AZCL-POLYSACCHARIDES FOR THE
ASSAY OF endo-HYDROLASES
11/99
AZCL-Polysaccharides （Azurine-Crosslinked Polysaccharides） are prepared by dyeing and
crosslinking highly purified polysaccharides.They are supplied as a fine powder （milled to pass
a 0.5 mm screen）.These substrates are insoluble in buffered solutions, but rapidly hydrate to
form gel particles which are readily and rapidly hydrolysed by specific endo-hydrolases releasing
soluble dye-labeled fragments.
These substrates can be used to locate enzyme activities in electrophoresis gels and to locate
specific enzyme-producing micro-organisms on culture plates. In tablet form, they can also be
used to measure enzyme activities in standard test-tube assays.
SUBSTRATES, APPLICATIONS and SUGGESTED BUFFER
SUBSTRATE ENZYME BUFFER
AZCL-Amylose a-Amylase （Fungal） Na acetate, 100mM, pH 4.4.
（Cereal） Na maleate, 100mM, pH 6.0.
（Bacterial） Bis-Tris, 100mM, pH 7.0.
AZCL-Barley ß-Glucan Malt ß-Glucanase Na acetate, 25mM, pH 4.5
Lichenase Na phosphate, 25 mM, pH 6.5.
Cellulase （Trichoderma） Na acetate, 25 mM, pH 4.5.
AZCL-HE-Cellulose Cellulase （Trichoderma） Na acetate, 25mM, pH 4.5.
Cellulase （Aspergillus） Na acetate, 25mM, pH 4.5.
AZCL-Curdlan & -Pachyman endo-1,3-ß-Glucanase Na acetate, 50mM, pH 6.0
AZCL-Debranched Arabinan endo-Arabinanase Na acetate, 50mM, pH 4.0.
AZCL-Dextran （No.B-512） endo-1,6-a-Dextranase Na acetate, 50mM, pH 5.0.
AZCL-Galactomannan endo-1,4-ß-Mannanase Na acetate, 50mM, pH 4.5.
AZCL-Galactan （potato or lupin） endo-1,4-ß-Galactanase Na acetate, 25mM, pH 4.3.
AZCL-Lichenan Malt ß-Glucanase Na acetate, 25mM, pH 4.5
Lichenase Na phosphate, 25 mM, pH 6.5.
AZCL-Pullulan Malt Limit-dextrinase Na maleate, 100mM, pH 5.5.
Microbial pullulanase Na acetate, 100mM, pH 5.0
AZCL-Xyloglucan （Tamarind） Cellulase （Trichoderma） Na acetate, 25 mM, pH 4.5.
AZCL-Xylan （Birch or oat） endo-Xylanase Na acetate, 25mM, pH 4.7.
AZCL-Arabinoxylan （wheat） endo-Xylanase Na acetate, 25mM, pH 4.7.
AZCL-Casein endo-Protease Na phosphate, 100mM pH 7.0.
AZCL-Collagen endo-Protease Na phosphate, 100mM pH 7.0.
NOTE: For use in agar plates, a concentration of 0.1% of AZCL-Polysaccharide is suggested.
CONDITIONS OF USE OF AZCL-POLYSACCHARIDES:
AZCL-Polysaccharides are the active ingredient in the test tablets supplied by Megazyme. Consequently,
further information on possible applications and assay conditions for these
substrates can be obtained by reference to the appropiate Test Tablet booklet. In general, the concentration
of the AZCL-polysaccharide in the test tablet is 30% w/w.
AZCL-Polysaccharide Test Tablet For the Measurement of:
AZCL-Amylose Amylazyme a-Amylase
Amylazyme BG a-Amylase
AZCL-Barley ß-Glucan Beta-Glucazyme Malt ß-Glucanase
Lichenase
Cellulase （Trichoderma）
AZCL-HE-Cellulose Cellazyme C Cellulase （Trichoderma）
Cellulase（Aspergillus）
AZCL-Curdlan & -Pachyman 1,3-Beta Glucazyme endo-1,3-ß-Glucanase
AZCL-Debranched Arabinan Arabinazyme endo-Arabinanase
AZCL-Dextran （No.B-512） Alpha-Dextrizyme endo-1,6-a-Dextranase
AZCL-Galactomannan Beta-Mannazyme endo-1,4-ß-Mannanase
AZCL-Galactan （potato or lupin） Galactazyme endo-1,4-ß-Galactanase
AZCL-Lichenan （not in tablet form） Malt ß-GlucanaseNa acetate,
Lichenase
AZCL-Pullulan Limit-Dextrizyme Malt Limit-dextrinase
Microbial pullulanase
AZCL-Xyloglucan （Tamarind） Cellazyme T Cellulase （Trichoderma）
AZCL-Xylan （Birch or oat） （refer to Xylazyme AX） endo-Xylanase
AZCL-Arabinoxylan （wheat） Xylazyme endo-Xylanase
Xylazyme AX endo-Xylanase
AZCL-Casein Protazyme AK endo-Protease
AZCL-Collagen Protazyme OL endo-Protease
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